Figure 5. Co-culture with rIL-10 amplifies cytokine production and restores cytokine production capacity in IL-10−/− BMMCs.
WT and IL-10−/− BMMCs were cultured in the presence or absence of rIL-10 for 24 hours (A–D) or 7 days (E–F). BMMCs were activated with IgE and antigen for 12 hours and cytokine levels were enumerated in cell culture supernatants by ELISA. Data are representative of 3 or more independent experiments. *=p<0.05; **=p<0.01. (student’s t-test).