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. 2016 Mar 30;5:416. [Version 1] doi: 10.12688/f1000research.8154.1

Figure 1. Identification, by immunoblots, of the epitope recognized by anti-α-SKA (A) and anti-α-CAA (B).

Figure 1.

Purified α-SKA ( A) or α-CAA ( B) was run on 10% SDS-PAGE and transferred on nitrocellulose membrane. Membrane strips were incubated with the mAb anti-α-SKA ( A) or anti-α-CAA ( B) alone (control, lane 1) or mixed with the listed peptides (2–9). The epitope recognized by anti-α-SKA ( A) includes the acetyl group and the first 7 amino acids of the α-SKA sequence. The epitope recognized by anti-α-CAA ( B) includes the acetyl group and the first 5 amino acids of the α-CAA sequence.