Table 3. Details of immunofluorescence staining protocol.

Protocol steps	Reagent	Concentration /dilution	Time	Catalogue Number
• Cryopreserved Rat muscles    • Cryostat sections (3μm)
Fixation	• Paraformaldehyde    • Methanol	1% 100%	30 min at RT 5 min at –20°C
Staining	Primary Abs: MAbs:    • anti-α-SKA (10D2)    • anti-α-CAA (22D3)    • anti-α-SMA (1A4) For details, see Table 1    • anti-vimentin (clone V9) Immunogen: Purified vimentin from porcine eye lens Specificity: the antibody cross-reacts with vimentin in man, cow, dog, hamster, horse, rhesus and African green monkey, rabbit, rat and rat kangaroo. No cross-reaction with mouse vimentin could be demonstrated, and results on chicken specimens are contradictory. Rabbit polyclonal Ab:    • anti-α-CAA ( Clément et al., 2003)	1:50 1:50 1:50 1:300 1:100	2 h	Home made Home made Home made Dako #M 0725 Home made
	Secondary Abs (Jackson Immmunoresearch):    • AffiniPure Goat Anti-Mouse IgG1, Alexa 488    • AffiniPure Goat Anti-Mouse IgG1, Alexa 594    • AffiniPure Goat Anti-Mouse IgG2a, Alexa 488    • AffiniPure Goat Anti-Mouse IgG2a, Alexa 594    • AffiniPure Goat Anti-Rabbit-Alexa 594	1:200 1:200 1:200 1:200 1:100	1 h	115-545-205 115-585-205 115-545-206 115-585-206 111-585-144