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. Author manuscript; available in PMC: 2017 Jun 28.
Published in final edited form as: J Control Release. 2016 Apr 15;232:83–92. doi: 10.1016/j.jconrel.2016.04.019

Fig. 4. Localization of neutral NPs at site of tumor metastasis in bone.

Fig. 4

A) Bioluminescence signal due to PC-3M-luc prostate cancer cells at 7 days post inoculation as measured using IVIS® and B) micro-CT images of the bone at 7 days post cancer cell inoculation. C) Fluorescence imaging and D) quantification of signal intensity measured using Maestro, demonstrating greater localization of NPs in tibia with tumor than in normal contralateral tibia. E) Ex vivo image (by Maestro) of the bone with intraosseous tumor at 24 hrs following NP administration. Arrows indicate bone with metastatic tumors. F) Bioluminescence signals due to cancer cells and fluorescence of NPs, demonstrating localization of NPs into metastasized tumor mass. For the above colocalization study, both bioluminescence signals due to cancer cells and fluorescence signals due to NPs were captured using IVIS®. Bright spots seen next to the tumor in tibia (Fig. 4F, fluorescence signal) are due to localization of the injected NPs in lymph nodes and other tissues. Data are shown as mean ± s.e.m., *P < 0.05.