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. Author manuscript; available in PMC: 2017 Jun 15.
Published in final edited form as: J Immunol. 2016 May 13;196(12):5112–5120. doi: 10.4049/jimmunol.1502153

Figure 7. CM from PGE2-treated AMs inhibits LPS-induced STAT3 activation and MCP-1 expression in AECs.

Figure 7

AMs were treated with 1 μM PGE2 for 1 h. The resulting AM-CM was collected and cultured with AECs for 2 h. The AECs were washed and stimulated with 10 ng/mL LPS in serum-free RPMI for 1–2 h, then lysed and analyzed for phospho-STAT3 by WB or MCP-1 mRNA by real-time PCR. (A) Schematic of experimental design testing the effects of AM-CM on LPS-induced STAT3 activation in AECs. (B) Representative blot (top) and fold increase (bottom) of normalized AEC phospho-STAT3 compared to non-LPS treated control. (C) Fold increase of MCP-1 mRNA determined by qRT-PCR. Data are expressed as fold change relative to untreated control and represent mean ± SEM from three separate experiments, *= p<0.05.