Table 5. In silico sequence similarity analysis between chicken IgY and rabbit IgG and array-identified human proteins.
14 most similar human proteins detected in this study, prioritised by sequence similarity.
| Human protein | Chicken Immunoglobulins | Rabbit Immunoglobulins | Signal
Intensity |
||||||
|---|---|---|---|---|---|---|---|---|---|
| BLAST
overlaps * |
Highest
BLAST E-value |
SIM
overlaps * |
Highest
Score |
BLAST
overlaps * |
BLAST | SIM
overlaps * |
Highest
Score |
||
| Clone SFV019_2F0 5H
immunoglobulin heavy chain variable region |
1 | 1.00E-48 | 2 | 393 | 2 | 3.00E-47 | 4 | 356 | 3 |
| Clone IgA-MZ-aa42c-2
immunoglobulin alpha heavy chain variable region (IgA) |
1 | 4.00E-30 | 2 | 210 | 1 | 1.00E-29 | 1 | 194 | 3 |
| IGH mRNA for
immunoglobulin heavy chain VHDJ region, partial cds, clone:TRH1-16 |
2 | 2.00E-34 | 1 | 218 | 1 | 1.00E-41 | 2 | 291 | 3 |
| Immunoglobulin heavy
constant gamma 1 (G1m marker) |
1 | 3.00E-13 | 1 | 86 | 2 | 2.00E-125 | 2 | 890 | 3 |
| Clone IP80 immunoglobulin
heavy chain variable region |
1 | 3.00E-44 | 1 | 313 | 1 | 3.00E-49 | 2 | 357 | 2 |
| Enhancer of polycomb
homolog 1 (Drosophila)e |
0 | BT | 1 | 51 | 0 | BT | 0 | BT | 2 |
| Inverted formin, FH2 and
WH2 domain containing |
0 | BT | 0 | NA | 0 | BT | 1 | 51 | 1 |
| Chromosome 10 open
reading frame 114 |
0 | BT | 1 | 51 | 0 | BT | 0 | BT | 1 |
| RUN and SH3 domain
containing 2 |
0 | BT | 1 | 52 | 0 | BT | 0 | BT | 1 |
| Single stranded DNA
binding protein 4 |
0 | BT | 1 | 56 | 0 | BT | 0 | BT | 1 |
| Rho GTPase activating
protein 33 |
0 | BT | 1 | 54 | 0 | BT | 0 | BT | 1 |
| Splicing factor 3b, subunit 4,
49kDa |
0 | BT | 1 | 61 | 0 | BT | 0 | BT | 1 |
| Protein phosphatase 1,
regulatory subunit 26 |
0 | BT | 1 | 59 | 0 | BT | 0 | BT | 1 |
| Alpha tubulin
acetyltransferase 1 |
0 | BT | 1 | 50 | 0 | BT | 0 | BT | 1 |
‘BT’ signifies ‘Below Threshold Value’. Threshold values were BLAST E-values below 1 × 10 -10 and SIM values above 50. ‘NA’ signifies ‘No significant similarity was detected’
*BLAST and SIM overlaps indicate the number of sequence categories meeting threshold criteria for similarity as shown in Supplementary Table 1.