Figure 6. RIPK1 kinase activity and PGAM5 are required for in vivo control of Leishmania replication.

Wild type, Ripk1^kd/kd or Pgam5^−/− mice were infected with Leishmania amazonensis in the footpad. (a) Histology sections of infected footpad at 5 weeks post-infection were examined. Representative images were shown and revealed similar extent of inflammation marked by neutrophilic infiltration in WT, Ripk1^kd/kd and Pgam5^−/− mice. The boxed areas were magnified to show intracellular parasites in the vacuoles. (b) Parasite load in the footpad was determined 10 weeks after infection. (c–d) Lesion size on the footpad was monitored weekly. Two independent experiments. **p<0.01, ***p<0.05.