Table 3.

Results from minimised design experiments in five soil types showing average measured concentrations in E. fetida (n = 6) at the end of 21 d uptake phase (C_t1) and 21 d depuration phase (C_t2) and average concentration (n = 3) of pharmaceutical in the pore water during the uptake phase (C_pw) (±standard deviation). Calculated uptake (k₁) and depuration rates (k₂) are presented along with pore water based BCF values derived using the minimised design approach. Soil/water adsorption coefficients (K_d) are also provided with soil BSAF estimates based on K_d values.

Soil type		Ct1 Bq/g (internal)	Ct2 Bq/g (internal)	Cpw (Bq/mL)	k2 (dep. Rate) (d−1)		k1 (uptake rate) (mL/g d−1)		Pore water BCF	Soil Kd (average 21 d)	Soil BSAF
Carbamazepine
2.1	76.13 ± 13.33		1.49 ± 0.68	59.78 ± 14.61		0.187	0.24	1.30		1.34	0.97
2.3	27.94 ± 6.11		0.17 ± 0.03	18.37 ± 3.57		0.243	0.37	1.53		3.87	0.40
2.4	25.79 ± 2.94		0.28 ± 0.74	16.24 ± 2.27		0.215	0.35	1.61		4.45	0.36
5M	34.49 ± 3.09		0.52 ± 0.52	33.37 ± 5.58		0.200	0.21	1.05		2.20	0.48
6S	35.10 ± 4.82		1.22 ± 0.36	23.35 ± 6.03		0.160	0.25	1.56		3.44	0.45
Diclofenac
2.1	413.80 ± 166.08		233.65 ± 141.58	33.28 ± 13.72		0.027	0.77	28.56		6.88	4.15
2.3	34.12 ± 13.76		31.67 ± 12.88	31.50 ± 11.67		0.004	0.05	15.04		7.25	2.07
2.4	31.61 ± 9.61		26.64 ± 8.74	10.85 ± 1.92		0.008	0.15	18.53		18.37	1.01
5M	34.59 ± 3.52		34.14 ± 11.51	38.20 ± 14.80		0.001	0.04	69.57		5.63	12.36
6S	67.99 ± 11.95		42.29 ± 22.54	25.64 ± 9.93		0.023	0.16	7.02		6.37	1.10
Fluoxetine
2.1	61.94 ± 8.65		5.52 ± 0.96	3.33 ± 0.58		0.115	2.35	20.42		55.48	0.37
2.3	45.27 ± 5.81		7.82 ± 2.36	2.77 ± 0.42		0.084	1.65	19.74		64.85	0.32
2.4	22.46 ± 3.55		5.41 ± 1.78	2.10 ± 0.29		0.068	0.96	14.09		71.44	0.20
5M	34.70 ± 6.66		10.09 ± 3.69	2.55 ± 0.29		0.059	1.13	19.18		64.06	0.30
6S	28.91 ± 12.23		2.97 ± 0.81	1.91 ± 0.61		0.108	1.83	16.89		58.17	0.29
Orlistat
2.1	116.83 ± 19.03		57.15 ± 6.97	7.50 ± 3.22		0.034	1.04	30.50		28.99	1.05
2.3	56.91 ± 8.12		47.04 ± 9.52	2.83 ± 1.45		0.009	1.05	115.88		75.10	1.54
2.4	35.43 ± 6.14		26.03 ± 5.86	1.78 ± 0.78		0.015	1.10	74.95		110.01	0.75
5M	56.88 ± 15.30		26.61 ± 13.73	2.60 ± 1.58		0.036	1.49	41.13		84.59	0.49
6S	37.93 ± 7.12		32.68 ± 14.15	3.98 ± 1.37		0.007	0.49	68.86		51.30	1.34