Figure 1.

Lymphocyte proliferation response of splenic lymphocytes after antigen recall. After immunization course, splenocytes were harvested and re-stimulated in vitro with peptide for 72 h and lymphocyte proliferation was then quantitated using a commercially available Brdu proliferation kit as described in ‘Materials and methods’. Proliferation was presented as stimulation index of individual mice and values were represented as mean ± SD of experimental mice. Asterisks represent the groups which were significantly difference (p < 0.05).