Table 1.

Methylation-specific polymerase chain reaction (PCR) (MSP) primers used in the present study

Genes	Primer sequence (5′–3′)	Products size
CHFR Flank Up	AAAAATAAGGAAAAGATGTAGAYG	167
CHFR Flank Down	TAAAAATTTCCRATTAAAAAAACCC
CHFR Me-Sense	AGATGTAGACGTTTTTTTTTGGAGGC	100
CHFR Me-Anti-Sense	ATTTCCGATTAAAAAAACCCCTTAACG
CHFR Un-Sense	AGATGTAGATGTTTTTTTTTGGAGGT	115
CHFR Un-Anti-Sense	AAATTTCCAATTAAAAAAACCCCTTAACA
MGMT Flank Up	GYGTTTYGGATATGTTGGGATAGTT	135
MGMT Flank Down	AAACTCCRCACTCTTCCRAAAAC
MGMT Me-Sense	TTTCGACGTTCGTAGGTTTTCGC	81
MGMT Me-Anti-Sense	GCACTCTTCCGAAAACGAAACG
MGMT Un-Sense	TTTGTGTTTTGATGTTTGTAGGTTTTTGT	93
MGMT Un-Anti-Sense	AACTCCACACTCTTCCAAAAACAAAACA
FANCF Flank Up	GGTTTTAAGTATTATTTAYGTTAGTATTT	165
FANCF Flank Down	CCTCTTACCTCCACTAATTATACAAC
FANCF Me-Sense	AGTATTTGGGATTTCGTTATCGTGC	99
FANCF Me-Anti-Sense	GAATAAAACCATACCGACCAAAACG
FANCF Un-Sense	TATGTTAGTATTTGGGATTTTGTTATTGTGT	106
FANCF Un-Anti-Sense	ACAAATAAAACCATACCAACCAAAACA
MLH1 Flank Up	GGAGTGAAGGAGGTTAYGGGTAAGT	182
MLH1 Flank Down	AAAAACRATAAAACCCTATACCTAATCTATC
MLH1 Me-Sense	ACGTAGACGTTTTATTAGGGTCGC	115
MLH1 Me-Anti-Sense	CCTCATCGTAACTACCCGCG
MLH1 Un-Sense	TTTTGATGTAGATGTTTTATTAGGGTTGT	124
MLH1 Un-Anti-Sense	ACCACCTCATCATAACTACCCACA
RASSf1 Flank Up	GTTTAGTTTGGATTTTGGGGGAG	144
RASSf1 Flank Down	CCCRCAACTCAATAAACTCAAACTC
RASSf1 Me-Sense	GGGTTCGTTTTGTGGTTTCGTTC	76
RASSf1 Me-Anti-Sense	TAACCCGATTAAACCCGTACTTCG
RASSf1 Un-Sense	GGGGTTTGTTTTGTGGTTTTGTTT	81
RASSf1 Un-Anti-Sense	AACATAACCCAATTAAACCCATACTTCA