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. Author manuscript; available in PMC: 2016 Jun 6.
Published in final edited form as: J Biomed Mater Res A. 2014 Mar 3;102(12):4568–4580. doi: 10.1002/jbm.a.35122

FIGURE 5.

FIGURE 5

Histology showing DIC-, TRAP-, ALP-, and hematoxylin-stained sections of the critical sized defects after 4 weeks of implantation with Healos along with BMSCs, with or without rhBMP-2 Full calvarial sections (scale bars: 1000 μm), magnified images a–d (scale bar: 50 μm). DIC sections show activity of GFP reporters in the regenerated tissues colocalized with alizarin complexone (AC). Bright green host-derived cells as well as very few donor cell derived bright blue cells are observed, lined with a red mineralizing AC label. These are mature osteoblasts. Host-derived light green elongated cells form a periosteum-like layer is seen at the periphery of the defects. TRAP: Bright yellow stain colocalized with light green/blue cells represents TRAP positive osteoclasts. ALP: Red color indicates ALP positive cells. Cell nuclei are stained white with DAPI. (ˆ) Early woven bone with disorganized mineralized, ALP positive matrix; (*) host-derived fibroblastic periosteum-like layer.