Table 3.

Overall Rate Constant, k, and Calculated Absolute Rates of Reduction for 0.5 mM Ascorbic Acid with 100 µM Azurin Variants in 20 mM Sodium Phosphate Buffer pH 6.35

variant	rate constant, k (min/(mM Asc1/2))	abs reduction rate with 0.5 mM ascorbate ((mM Az)/min)
Az-WT	0.25(2)	0.017
Az-NiR	0.57(4)	0.043
Az-NiR3His	1.05(7)	0.083
Az-PHM	0.26(4)	0.024
Az-PHM3His	0.36(2)	0.021