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. 2015 Mar 10;5:8902. doi: 10.1038/srep08902

Figure 4. The effect of ARI and PAL on cAMP release in perifused rat pituitary cells.

Figure 4

(a) PAL increased cAMP release (20–80 min application) compared to the baseline levels (0–20 min). The washout of PAL was accompanied with a gradual but incomplete return of cAMP release to basal levels during the 20 min washout period. Basal indicates cAMP release in untreated cells (open diamonds). (b) ARI (open squares) decreased cAMP release less effectively than DA (open circles). Applying DA to cells pre-treated with ARI further inhibited cAMP production. In contrast, PAL increased cAMP release from cells (closed circles). The addition of DA diminished this increase to basal levels only. Applying PAL to cells pretreated with DA diminished the inhibitory effect of DA on cAMP release (open circles). (c) Application of forskolin, an adenylyl cyclase activator, tremendously facilitated cAMP release (open diamonds). ARI decreased forskolin-stimulated cAMP release (open squares) in a similar fashion but less effectively than DA (open circles), whereas PAL increased forskolin-stimulated cAMP production (closed circles). All drugs were applied at a 1 μM concentration. The vertical dotted line indicates the moment of switch in drug application as indicated above the line.