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. 2015 Mar 10;5:8773. doi: 10.1038/srep08773

Table 1. Dwell state kinetics.

Form   Michaelis Menten fits   Dwell analysis
  vmax (rev s−1) Km (μM) kATP (s−1 μM−1) n kATP (s−1 μM−1) k2 (ms)−1 k3 (ms)−1 n
Wild type 586 ± 24 62 ± 9 28.3 ± 3.6 2177 18 ± 6 2.5 ± 1.2 4.3 ± 2.3 18, 28
γG278D 582 ± 137 63 ± 33 27.6 ± 10.2 89 26 ± 2 2.6 ± 0.6 5.0 ± 1.7 4, 5
βV279F 144 ± 13 24 ± 5 17.9 ± 2.8 537 15 ± 2 0.36 ± 0.08 2.3 ± 2.7 4, 8
αN67I 248 ± 47 41 ± 12 18.0 ± 2.6 432 14 ± 3 0.46 ± 0.09 2.3 ± 0.7 7, 3
βR408G 80 ± 11 10.0 ± 2.6 24.1 ± 4.0 439 19 ± 5 0.21 ± 0.08 1.3 ± 0.5 10, 17
αF405S 336 ± 40 49 ± 11 20.4 ± 3.2 1044 16 ± 6 0.8 ± 0.6 2.0 ± 0.9 62, 40
γI270T 377 ± 92 44 ± 15 25.7 ± 4.4 2190 22 ± 7 0.8 ± 0.5 2.5 ± 2.0 109, 27
βR408I 69 ± 13 7.9 ± 2.5 26.4 ± 4.7 6519 23 ± 6 0.20 ± 0.07 3.3 ± 2.3 384, 51
Bacillus PS333 129 ± 22 15 ± 5 25.8          
E. coli6 449 ± 39 21.1 ± 1.0 43 ± 4          

The results of the analysis from the Michaelis-Menten fits and the dwell time histogram analysis for wild type and the seven mutants screened. All values are quoted with 95% confidence intervals, with the exception of Bacillus PS3 and E. coli, where the values were sourced from previous studies as indicated in the table. The errors in these studies were typically quoted as standard deviations; for comparison to the 95% CIs listed here they have been multiplied by 1.96 for data where the number of degrees of freedom were unknown, or by the appropriate t-statistic where the number of degrees of freedom was known (2.45 to 1.96 for df = 6 to ∞). In some cases the error could not be calculated from the available data and has been left blank. The number of traces used is denoted by n. For the dwell analysis, the first value of n corresponds to the number of traces used to calculate kATP and the second value for the number of traces used to calculate k2 and k3.