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. 2014 Jun 17;4:5290. doi: 10.1038/srep05290

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(a) Primers designed to genotype the Fah mutant allele by PCR. Primers FAH-F1, FAH-R1 and FAH-probe are used in the same PCR reaction. Primers FAH-F1 and FAH-R1 amplify a 560 bp segment from the WT and 565 bp from the STOP-BamHI knock-in allele. The primers FAH-F1 and FAH-probe, specifically targeting the knock-in sequence, only amplify a 454 bp segment. (b) PCR genotyping results of F1 generation mice. The mouse numbers, from F0001 to F0010, are shown above each lane. Founder #1-1 and wild type mouse genomic DNA serve as positive and negative controls, respectively. Blank is a reaction without any DNA template. The amplicon size is shown on the right. The gender of each mouse is shown below each lane. ♀, female; ♂, male. Left lane, DNA ladder. Cropped gel is shown. Full-length gel is provided for review in the supplementary file.