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. 2015 Apr 1;5:9559. doi: 10.1038/srep09559

Figure 6. Effects of knockdown of TLR pathway genes on specific ligand responses in mouse and human macrophage reporter cell lines.

Figure 6

(a) Human TNF-α reporter responses in THP1 B5 cells transfected with either non-targeting control or TLR pathway gene-specific siRNAs and treated for 4 hr with either 10 ng/ml LPS, 10 μg/ml R848 or 10 ng/ml FLG. (b) tnf promoter-driven mCherry expression at 16 hr and (c) Cytosol-to-nuclear translocation of the GFP-relA fusion at 40 min in RAWG9 cells transfected with either non-targeting control or TLR pathway gene-specific siRNAs and treated with 10 ng/ml LPS or 3 μM R848. 6 individual siRNAs per gene were used (Supplementary Table 1) and the average reporter response calculated. Data are representative of two experiments and reporter responses are normalized to the levels observed with non-targeting control siRNA (a–c; mean + s.d.). **P < 0.01, ***P < 0.001, ****P < 0.0001 (two-tailed t test).