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IHC double stains showing the FAT10and ubiqutin of a liver from a FAT10 wild type strain control mouse fed the control diet with DDC added for 10 weeks. A) FAT10 antibody filter (FITC). B) Ubiquitin antibody filter (Texas red). C) Tricolor filter. Multiple ubiquitin positive MDBs are present (arrows) D) The same liver stained with Hematoxylin and eosin showing changed liver histology. X225
