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. 2016 Jun 6;11(6):e0156857. doi: 10.1371/journal.pone.0156857

Fig 5. Effect of vitamin D supplementation on VSMC proliferation after balloon angioplasty.

Fig 5

Photomicrographs showing immunohistochemical expression of proliferating cell nuclear antigen (PCNA) in thin sections of post-balloon angioplasty coronary arteries from vitamin D-deficient high cholesterol diet (panels A-B) and vitamin D-sufficient high cholesterol diet + oral vitamin D supplemental swine (panels C-F). Sections were stained with DAB as chromogen and counterstained using hematoxylin. Arrows indicate cells expressing PCNA. Scale bar 100 μm; L: lumen, M: medial layer, NI: neointima; Data are shown as mean ± SEM (N = 4); *p< 0.05, **p< 0.01, ***p< 0.001.