Table 1. Experimental sensitivity, specificity and accuracy of functional assays and siRNA screening using the experimental best cut-off.
| Standard method | MWW method | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Assay | Best cut-off | Youden's index | Sensitivity | Specificity | Accuracy | Best cut-off | Youden's index | Sensitivity | Specificity | Accuracy |
| Colony Size | 17 910 | 0.89 | 24/25 = 0.96 | 14/15 = 0.93 | 38/40 = 0.95 | 0.009 | 0.89 | 24/25 = 0.96 | 14/15 = 0.93 | 38/40 = 0.95 |
| Liquid Medium | 0.172 | 0.81 | 22/25 = 0.88 | 14/15 = 0.93 | 36/40 = 0.90 | 0.00023 | 0.80 | 20/25 = 0.80 | 15/15 = 1.00 | 35/40 = 0.88 |
| Spot Formation | 0.295 | 0.77 | 21/25 = 0.84 | 14/15 = 0.93 | 35/40 = 0.88 | 0.165 | 0.77 | 21/25 = 0.84 | 14/15 = 0.93 | 35/40 = 0.88 |
| Yeast Localization | 0.057 | 0.69 | 19/25 = 0.76 | 14/15 = 0.93 | 33/40 = 0.83 | 0.186 | 0.69 | 19/25 = 0.76 | 14/15 = 0.93 | 33/40 = 0.83 |
| siRNA | 604 | 1.00 | 1/1 = 1.00 | 2/2 = 1.00 | 3/3 = 1.00 | 0.00044 | 1.00 | 1/1 = 1.00 | 2/2 = 1.00 | 3/3 = 1.00 |
"Accuracy" is the number of mutations or siRNA correctly classified. Sensitivities, specificities and accuracies, associated with the experimental best cut-off, were referred to as "experimental", to distinguish them from those computed using the probability system of variant classification.