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. 2016 Jun 6;11(6):e0156929. doi: 10.1371/journal.pone.0156929

Fig 1. Influenza A virus induces the release of BPI from human granulocytes.

Fig 1

After Dextran sedimentation granulocytes were isolated from human buffy coats by density gradient centrifugation. After the control of the purity of the collected neutrophils by the measurement of the surface expression of CD14 and CD66 using flow cytometry the degranulation of the granulocytes were induced in the presence of purified Influenza A virus subtypes A) H1N1, B) H3N2 and C) H5N1 (0.5, 1, 2, 4, and 8 MOI, black bar) or left unstimulated (white bar). The IAV strain rg A/Vietnam//1203/04 (H5N1) harbours only hemagglutinin as well as neuraminidase form H5N1 and the rest of the virus is of strain A/PR/8/34 origin. After 4 as well as 16 hrs of stimulation the supernatants were collected and the content of BPI in the supernatant was determined via ELISA. Three experiments with similar results were performed and one representative is shown. Statistically significant differences are given as p values (** <0.01); n = 3 ± SEM.