Table 1. Performance of proposed and the state-of-the-art methods.

	Proposed	Ilastik	FARSight	RPHC	3D watershed	CellSegmentation3D
TP	184.92	169.67	155.25	141.67	161.17	152.33
FP	9.42	5.08	3.92	11.50	22.08	40.33
FN	6.00	21.25	35.67	49.25	29.75	38.58
Found	194.33	174.75	159.17	153.17	183.25	192.67
TP rate	0.9686	0.8885	0.8133	0.7419	0.8440	0.7981
FP rate	0.0493	0.0265	0.0205	0.0602	0.1158	0.2115
FN rate	0.0314	0.1115	0.1867	0.2581	0.1560	0.2019
F-measure	0.9602	0.9277	0.8865	0.8232	0.8615	0.7943
Accuracy	0.9239	0.8657	0.7969	0.7001	0.7572	0.6597
Time (sec)	202.60	240.75	14.73	537.41	40.98	352.70

The means of 12 animals are shown. TP, FP and FN mean True Positive, False Positive and False Negative, respectively. The mean of the Grand Truth (GT) is 190.92. The details of performance indices are described in Methods section.