Table 1. Relationship between the length of G1 in log phase and high density Q cell yield.
Strain | %G1 | SD | %Q | SD | |
---|---|---|---|---|---|
BY5654 | 5xCLN3 | 6.8 | 1.5 | 26 | 5.4 |
BY6828 | sic1 | 6.8 | 0.0 | 19 | 2.3 |
BY6698 | 5xCLN3rad53-21 | 7.2 | 0.9 | 1.4 | 0.0 |
BY7002 | swi6 | 7.8 | 4.3 | 0.1 | 0.2 |
BY7326 | whi5 | 10.3 | 0.5 | 45.2 | 2.4 |
BY7794 | stb1whi5 | 11.5 | 0.2 | 40.0 | 3.6 |
BY7334 | whi5srl3 | 12.3 | 0.4 | 46.6 | 0.8 |
BY7140 | msa2rad53-21 | 13.0 | 0.3 | 54.7 | 2.1 |
BY7171 | msa2rad53-21 | ||||
BY6848 | rad53-21 | 14.5 | 5.0 | 52 | 4.4 |
BY7793 | stb1msa2 | 17.5 | 2.1 | 40.8 | 8.0 |
BY7067 | msa1rad53-21 | 17.9 | 3.2 | 62 | 1.3 |
BY7448 | whi5msa1 | 17.9 | 0.2 | 56.7 | 4.9 |
BY7795 | stb1msa1 | 17.9 | 0.2 | 38.9 | 0.4 |
BY7146 | xbp1rad53-21 | 18.6 | 1.0 | 46 | 2.6 |
BY6884 | stb1 | 19.1 | 0.5 | 62 | 1.6 |
BY6879 | msa1 | 19.2 | 0.7 | 61 | 2.9 |
BY6966 | hog1 | 19.2 | 0.2 | 51 | 8.1 |
BY6883 | msa1msa2 | 19.6 | 1.6 | 34 | 6.4 |
BY6602 | xbp1 | 20.1 | 0.9 | 54 | 3.7 |
BY7332 | srl3 | 21.0 | 1.0 | 62.1 | 1.9 |
BY7454 | whi5msa2 | 21.1 | 0.5 | 68.6 | 2.0 |
BY6500 | WT | 21.2 | 2.1 | 56 | 8.6 |
BY6881 | msa2 | 21.3 | 0.9 | 57 | 1.5 |
BY7131 | xbp1cln3 | 28.4 | 0.0 | 60 | 1.5 |
BY7026 | mbp1 | 29.0 | 0.7 | 53 | 2.5 |
BY6873 | cln3 | 34.3 | 1.9 | 70 | 1.2 |
Strains are all isogenic with prototrophic W303 (BY6500) with relevant genotype listed. Percent of cells in G1 during log phase and the percent yield of high density (Q) cells after seven days of growth are listed, with standard deviations (SD) calculated from 2 to 6 measurements, except sic1 and xbp1cln3, which were only measured once. Two independently derived msa2rad53-21 strains were analyzed because of the variability observed with this strain. Swi4 is not included because it is lethal in the BY6500 background.