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. 2016 Jun 6;11(6):e0156716. doi: 10.1371/journal.pone.0156716

Fig 2. Epitope mapping of Ra48 mAb.

Fig 2

(A) Identification of epitope region using DRD1 C-terminal fragments. The full (337–447) or partial fragments (337–376, 374–413, and 411–447) of the C-terminal cytoplasmic region of human DRD1 were fused to the C-terminus of bls-SrtA protein and synthesized using wheat germ cell-free system. Binding between the fusion protein and Ra48 antibody was detected by AlphaScreen. (B) Comparison of amino acid sequences between human DRD1 (374–413), mouse Drd1 (374–413), and rabbit DRD1 (376–415). Yellow denote the amino acids different from ones of human DRD1. (C) Immunoblot analysis of deletion mutants to identify the minimum Ra48 epitope sequence.