Fig 8. RbdB GFP fusion proteins fully complement the mutant phenotype.

Independent rbdB- strains that were transformed with the plasmid pDbsr2a RbdB GFP [LC, low copy] or pDneo2a RbdB GFP [HC, high copy] were analyzed with respect to miRNA expression A: miRNA expression were analyzed by Northern Blot. 12 μg total RNA were loaded per lane. One clone with low RbdB GFP expression and one clone with high expression each are shown. As controls, we loaded RNA from the AX2 wt and from rbdB- strains. Mature miRNAs were detected by specific ³²P labelled probes as described in Fig 3A. As a loading control, the membrane was finally rehybridized with a probe directed against the snoRNA Ddr6. B: miRNA signals from independent Northern Blots were quantified relative to the loading control and normalized to the AX2 wild type. According to paired t-test, no significant difference in expression levels was observed between the wild type and the different rescue strains.