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. 2016 Jun 6;11(6):e0156818. doi: 10.1371/journal.pone.0156818

Fig 1. Cytotoxicity of non-thermal plasma on prostate DU145 cancer and PrEC normal cells.

Fig 1

The cells were exposed to PBS treated with different plasma doses for 1 and 10 minutes, followed by dilution with growth medium and incubation for 24 and 48 hours. To simplify the understanding of non-thermal plasma doses, the plasma obtained at different physical parameters (Table 1) was named numerically as Dose 1, 2, etc. As seen from the graphs A and D the highest cytotoxic dose was Dose 7 (D7). (B,E) The quantitative graphs of the plasma induced cytotoxicity for both prostate cancer and normal cells. (C,F) The data and images obtained from the colony formation assay. The benign PrECs treated with plasma D7 for 1 and 10 minutes followed by PBS dilution with growth medium were cultivated for 6 days. They retain their proliferative activity while the cancer cells treated in the same way lose the ability to form colonies. Data presented as mean±SEM (n = 3).