Fig 6. Plasma induced modulations of cytosolic calcium in PrEC and DU145 cells.

Representative confocal microscopy spectral records of DU145 cells. After 10 minutes of incubation with or without plasma treated PBS cells were challenged with 50μM ATP. (A) IP₃-mediated intracellular calcium oscillations induced by ATP were produced in control cells. (B) In plasma treated DU145 cells this amount of ATP provoked sustained cytosolic calcium response, while non-thermal plasma treated PBS itself did not cause calcium modulations. In PrECs the high calcium signal was observed right after addition of non-thermal plasma treated PBS (D7) (C). The representative original records demonstrate the data collected from about 50 cells evaluated in each of 5 experiments.