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. 2016 Jun 6;11(6):e0157015. doi: 10.1371/journal.pone.0157015

Fig 3. CT-treated BMDCs downregulate T cell activation and IL-2 production.

Fig 3

OT-II CD4+ T cells were co-cultured with BMDCs or CT-BMDCs pulsed with the indicated concentration of OVA323-339 peptide. At 16 h, OT-II CD4+ T cells were analyzed for expression of CD25 and CD69 (A-C). Histogram of CD25 and CD69 (A); Frequency of CD25-expressing or CD69-expressing CD4+ T cells (B); Intensity of CD25 or CD69 expression (C). CFSE-labeled OT-II CD4+ T cells were cultured alone or with BMDCs or CT-BMDCs pulsed with the indicated concentration of OVA323-339 peptide. At day 4, CFSE dilution was assessed in OT-II CD4+ T cells (D). At day 5, IL-2 was detected in the co-culture of OT-II CD4+ T cells with BMDCs or CT-BMDCs by cytokine bead assay (eBioscience) (E). ***p<0.001 (Student’s t-test). Histograms and data are from one experiment representative of two independent experiments. Data are average ± SEM of triplicate wells.