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. 2016 Jun 6;11(6):e0156871. doi: 10.1371/journal.pone.0156871

Fig 2. Overexpression of miR-205 induces increased mucin production, while overexpression of miR-373 causes dedifferentiation.

Fig 2

(A-D) Caco-2WT overexpressing miR-205 or miR-373 display significant morphologic changes when compared to control Caco-2WT/miR-c. In contrast, suppression of miR-373 reverses some of the neoplastic characteristics of Caco-2D299G. Representative images (n ≥ 2 samples/clone) showing (A) H&E staining (bar, 200μm), (B) PAS (bar, 200μm), (C) TEM (bar, 5μm) and (D) immunofluorescent staining with anti-MUC2 (AlexaFluor® 647; yellow) and DAPI (blue), assessed by optical sectioning microscopy (bar, 50μm), are shown. Black star indicates vesicle (C); white arrows indicate mucus (B) or MUC2 (D) -positive structures.