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. 2016 Jun 6;11(6):e0156871. doi: 10.1371/journal.pone.0156871

Fig 3. Overexpression of miR-205 and miR-373 differentially alter morphological features of intestinal epithelial barrier integrity.

Fig 3

Expression of miR-205 and miR-373 differentially (A) alters actin cytoskeletal architecture, influences (B) ZO-1 and (C) phospho-β-CATENIN -associated barrier integrity, and (D) induces formation of multinucleated cells with multipolar spindles. Representative images (n ≥ 2 samples/clone) of immunofluorescent staining with (A) phalloidin (AlexaFluor® 647; white; yellow bar, 20.29μm [3-dimensional distance], white bar, 100μm), (B) anti-ZO-1 (AlexaFluor® 647; white; bar, 50μm) and DAPI (blue), (C) phospho-β-CATENIN (AlexaFluor® 647; green; bar, 50μm) and DAPI (red) and (D) anti-phospho-HISTONE H3 (PacificBlue® 350; green; bar, 100μm) and anti-β-TUBULIN (AlexaFluor® 647; red), as assessed by optical sectioning microscopy. (B)—(C) White arrows indicate exemplary regions of interests (magnified insets), as mentioned in Results. Representative stack scanning (33 stacks; Z-stack depth, 1μm) is shown in (A).