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. 2016 Jun 6;11(6):e0156871. doi: 10.1371/journal.pone.0156871

Fig 4. Overexpression of miR-205 drives goblet cell expansion and modulates cell cycle regulation.

Fig 4

miR-205 induces expression of distinct molecules related to goblet cell differentiation and cell cycle regulation, as assessed by (A) western blot, (B) qRT-PCR and (C) IPA analysis. (A) Representative results of at least 2 independent experiments are shown. Each individual membrane was reprobed with anti-GAPDH to confirm equal loading and one representative blot is shown. (B) Results (log2 base) are shown in relation to mRNA expression for the housekeeping gene GAPDH and normalized to the average expression of the corresponding vector controls. Data (n = 3 samples/clone) are presented as means ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; unpaired t-test). (C) The identified molecules were connected into a hypothetical common signaling model and associated with their main biological functions in the IPA knowledge base.