Fig 5. Overexpression of miR-373 induces signaling mediators of inflammation-associated progression and invasion.

miR-373 activates molecules related to inflammation and invasion, as assessed by (A) western blot, (B, D) qRT-PCR, (C) ELISA and (E) IPA analysis. (A) Representative results of at least 2 independent experiments are shown. Each individual membrane was reprobed with anti-GAPDH to confirm equal loading and one representative blot is shown. (B) Results (log2 base) are shown in relation to mRNA expression for the housekeeping gene GAPDH and normalized to the average expression of the corresponding vector controls. (C) Induction of protein secretion of pro-inflammatory factors (CHI3L1, TFPI) mediated by miR-373, as assessed by ELISA. (D) Conditioned media (cM) from Caco-2^D299G induces increased expression of miR-373 in Caco-2^WT, as assessed by qRT-PCR. Results of 2 independent experiments are shown in relation to RNU6 miRNA expression. (E) The identified molecules were linked within a hypothetical common signaling model and associated with their main biological functions in the IPA knowledge base. (B-D) Data (n = 3 samples/clone) are presented as means ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; unpaired t-test).