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. 2016 Jun 6;11(6):e0156871. doi: 10.1371/journal.pone.0156871

Fig 6. miR-205 and miR-373 drive different functions of colon cancer progression.

Fig 6

Functional analysis of miR-205 and miR-373 in colon cancerogenesis. miR-373 increases (A) loss of cell adhesion, (B) invasion and (C) proliferation, while miR-205 mediates (D) chemoresistance, as determined by (A) differential trypsinization, (B) Matrigel® invasion and (C+D) MTS assays, respectively. (A) Representative phase-contrast images of clones (Mayer’s hemalum solution) before and after 2min of trypsin treatment (n ≥ 3 samples/clone) are shown (bar, 50μm). (B) Shown are representative areas of the reverse side of the Matrigel® culture insert (n ≥ 2 samples/clone) stained with crystal-violet (bar, 100μm). (C) Results (log2 base) of 4 independent experiments are shown as proliferation ratio between day 3 and day 1, normalized to average of corresponding vector control. (D) Clones were treated with 10μM methotrexate (MTX) or vehicle (saline) control for 3 days. Results (log2 base) of at least 3 independent experiments are shown as proliferation ratio between day 3 and day 1 of MTX-treated cells in relation to vehicle control, normalized to average of corresponding vector control. (C+D) Data (n = 6 samples/clone) are presented as means ± SEM (vs. corresponding vector control: *p < 0.05, **p < 0.01, ns: not significant; unpaired t-test).