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. Author manuscript; available in PMC: 2017 May 4.
Published in final edited form as: Neuron. 2016 Apr 14;90(3):581–595. doi: 10.1016/j.neuron.2016.03.017

Figure 4. DmSLC22A knockdown and overexpression in the MB.

Figure 4

(A–B″). Representative of whole brains co-labeled with anti-Brpnc82 (magenta) and anti-DmSLC22A (green) antibodies, for control and 238Y-gal4 driven knockdown flies. The region of interest (ROI) for quantification is illustrated in panels F″ and G″. (C) The signal from the calyx in each fly was normalized to the signal in the optic lobes and the fluorescence intensity ratio (calyx intensity)/(optic lobe intensity) for the control genotype was set to 1.0. The fluorescence intensity ratio was reduced by ~43% and 30% in DmSLC22A knockdown flies driven by 238Y-gal4 and R13F02-gal4, respectively. Statistics: Two-tailed student’s t-test with ***P<0.001. N≥14. (D–E‴) Representative of whole brains co-labeled with anti-nc-82 (magenta) and anti-DmSLC22A (green) for control and 238Y-gal4 driven overexpressing flies. The region of interest (ROI) for quantitation of the signal is illustrated in panels I‴ and J‴. (F) The normalized signal increase in 238Y-gal4 driven DmSLC22A overexpression flies was ~2.5 fold. Statistics: Two-tailed student’s t-test with ***P<0.001. N=10. Scale bar = 20μm in A–B″ and D–E‴.