FIG 7 .

Tamoxifen induces accumulation of LC3-GFP on the PVM in murine cells. (A) MEFs were transfected with the LC3-GFP expression construct and then either mock or parasite infected. After 2 h, tamoxifen (Tmx; 5 µM) or DMSO was added and the cells were grown for an additional 24 h and then fixed and stained to detect SAG1. Toxo, Toxoplasma. (B) Western blot analysis shows levels of LC3-GFP expression and the extent of autophagy induction. Shown is a blot representative of three independent experiments. (C) Quantification of LC3-GFP accumulation on the PVM in parasite-infected cells that were treated with either DMSO or tamoxifen. *, P < 0.05 (paired Student t test).