TABLE 2 .
Transformation of recA+ and recA mutant strains carrying R388::IS26
| TU (transposase) |
Plasmid in UB1637 | Expt no. or parameter |
No. of Kmr Tpr transformants/µg of TU for strain: |
|
|---|---|---|---|---|
| UB1637 (recA) | E294 (recA+) | |||
| TUaphA1a (Tnp26) |
R388::IS26 | 1 | 100 | 195 |
| 2 | 272 | 126 | ||
| 3 | 153 | 181 | ||
| Avg | 175 | 167.3 | ||
| TUaphA1a (Tnp26-FS-La) |
R388::IS26 | 1 | 1 | 2 |
| 2 | 2 | 0 | ||
| 3 | 0 | 0 | ||
| 4 | 1 | 2 | ||
| Avg | 1 | 1 | ||
| TUaphA1a
(Tnp26) |
R388::IS26-FS-Rb | 1 | 2 | 1 |
| 2 | 2 | 2 | ||
| 3 | 1 | 0 | ||
| 4 | 1 | 2 | ||
| Avg | 1.5 | 1.3 | ||
| TUaphA1a
(Tnp26-FS-L) |
R388::IS26-FS-R | 1 | 0 | 1 |
| 2 | 0 | 2 | ||
| 3 | 0 | 1 | ||
| 4 | 0 | 2 | ||
| 5 | 0 | 0 | ||
| Avg | 0 | 1.5 | ||
a
IS26-FS-L, frameshift mutation in tnp26 in the TU, producing a truncated 30-aa protein.
b
IS26-FS-R, frameshift mutation in R388::IS26 tnp26.