Expression of PgcvH2::lacZ is induced with glycine in P. aeruginosa PAO1. P. aeruginosa wild-type (WT) PAO1, ΔgcsR PAO1, or ΔrpoN PAO1 cells harboring the PgcvH2::lacZ reporter construct was grown in M9 minimal medium to an OD600 of ~0.3 and then challenged with 10 mM glycine, 10 mM glutamate, 10 mM serine, or no substrate. Data points represent mean values ± the standard deviations (n = 3). Analysis of variance was performed by using Dunnett’s post hoc test (α value of 0.05) to identify significant differences (P < 0.0001; marked with an asterisk).