FIG 6 .
GcsR binds to three 18-bp tandem repeats in the gcvH2 promoter region. EMSAs were performed with His6-GcsR and 2 nM Cy5-labeled probe DNA. (A) Sequence of the 217-bp region upstream of the gcvH2 gene. The three 18-bp GcsR binding sites are in bold type. The underlined nucleotides in the GcsR binding sites were mutated to G in this study. The RpoN binding site is in bold italic type. (B) A 200 nM concentration of His6-GcsR was incubated with PgcvH2 (lane 2) or PgcvH2-mut1 (lane 4). (C) PgcvH2-2, PgcvH2-2-mut, PgcvH2-3, and PgcvH2-3-mut were incubated in the absence (lanes 1, 3, 5, and 7, respectively) or presence (lanes 2, 3, 6, and 8, respectively) of 200 nM His6-GcsR. (D) Consensus sequence of the GcsR binding site derived from the three 18-bp tandem repeats in the PgcvH2 sequence constructed with WebLogo 3.4.