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. 2016 Apr 27;1(2):e00020-16. doi: 10.1128/mSphere.00020-16

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Copyright © 2016 Sarwar et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 8 — GcsR affects pyocyanin production through the MexEF-OprN efflux pump. (A) Overexpression of rhlI or deletion of the mexF gene inactivating the MexEF-OprN pump in the gcsR::Tn mutant strain PW5126 restores pyocyanin production in PW5126. The ΔgcsR PAO1 strain produces pyocyanin levels similar to those of PAO1. Data points represent mean values ± the standard deviations (n = 3). Analysis of variance was performed by using Dunnett’s post hoc test (α value of 0.05) to identify significant differences (P < 0.0001; marked with an asterisk). (B) The P_mexE probe was incubated in the absence (lane 1) or presence (lane 2) of 200 nM His₆-GcsR.