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HCQ enhances antibiotic efficacy against intramacrophage AIEC. Combination therapy (antibiotic plus HCQ, 1 μg/mL) was more effective at killing AIEC HM605 within J774.A1 macrophages than antibiotic monotherapy at (A) 10% Cmax doxycycline and (B) Cmax doxycyline [2 μg/mL]; N = 6. Likewise, for (C) 10% Cmax ciprofloxacin. D, No additional benefit was seen with ciprofloxacin at Cmax [4 μg/mL]; N = 3. *P < 0.05, ***P < 0.001, ***P < 0.001; analysis of variance.
