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. Author manuscript; available in PMC: 2016 Jun 6.
Published in final edited form as: ACS Chem Biol. 2015 Aug 7;10(10):2334–2343. doi: 10.1021/acschembio.5b00452

Figure 2.

Figure 2

Overview of screening assay for OGG1 inhibitors. (a) Schematic of the fluorescence-based OGG1 inhibitor assay used for the inhibitor screen. Image adapted from ref 40. (b, c) OGG1 dose (1–20 nM) and kinetic data for the fluorescence-based (b) and the gel-based (c) assays showing similar kinetics. Relative percent fluorescence equals the percent fluorescence in the experimental well compared with a well containing only the TAMRA strand of the substrate. Percent cleaved product equals the cleaved product (p)/(uncleaved substrate (u) + p). Data points equal the mean of three independent experiments. The uncertainties are standard deviations. (d) Z′ values for each of the 156 plates screened for OGG1 inhibition. Ź values were calculated using fluorescence values from no inhibitor and no enzyme control wells on each plate.