Figure 4.

Phosphate medium induces increased protein expression of UT. SMCs were incubated under one of the following conditions: DMEM alone, DMEM + 100 nM UII, phosphate medium (Pi) alone or phosphate medium (Pi) + 100 nM UII. Two different degree of glycosylated UT (Upper-band and Lower-band) were detected around 60KD in SMCs. Phosphate medium significantly increases the total UT and Upper-band compared to DMEM and DMEM + UII conditions, ^*P < 0.01. UII stimulation significantly increased UT expression in Pi medium compared to Pi medium alone ^**P < 0.001. N = 4 for each condition. (A) Western blot visualization. (B) Relative UT quantification normalized to the expression of GAPDH.