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Extent of cell death and ROS generation in individual and combined stressed plants. Cell death was estimated at 72 hpt by trypan blue staining and photographs were taken (A). Stained area was scored by ImageJ software (http://imagej.nih.gov/ij/) and was expressed as fold change over control. DAB stained leaves showing ROS are presented (B). Each experiment was carried out with three biological replicates.
