Figure 1.

Root hairs isolation from Medicago truncatula. (A) Five-day old seedling just before root hairs isolation (Scale bar = 1 cm). Root hairs were isolated by vortexing frozen excised root segments long of about 4 cm and free from the root tip (0.5 cm) as displayed by the dotted line rectangle. (B,C) Representative photographs of root material before (B) and after (C) root vortexing, showing the absence of root hairs in the remaining material at the end of the isolation procedure (Scale bar = 100 μm).