Table 7.
Common proteins identified among the six PPD preparations.
| Identified Proteins (16/561) | Accession Number | Molecular Weight | PPD-A | PPD-B | PPD-F | PPD-K | PPD-M | PPD-N | Function | References |
|---|---|---|---|---|---|---|---|---|---|---|
| 10 kDa culture fíltrate antigen EsxB*, Mycobacterium tuberculosis | A2VMP9_MYCTX | 11 kDa | + | + | Immunodominant antigen involved in secretion, comple × formation, immunogenicity and virulence | Brodin et al., 2005 | ||||
| 10 kDa chaperonin GroES*, Mycobacterium tuberculosis | A2VPK5_MYCTX | 11 kDa | + | + | + | + | + | Immunodominant protein, ATP binding | Uniprot, Prasad et al., 2013 | |
| 60 kDa chaperonin GroEL*, Mycobacterium tuberculosis |
R4LUN2_MYCTX A4KEC8_MYCTX |
57 kDa | + | + | + | + | + | + | Immunodominant protein | Uniprot, Prasad et al., 2013 |
| 50S ribosomal protein L7/L12, Mycobacterium parascrofulaceum | D5PC72_9MYCO | 13 kDa | + | + | + | Involved in interaction with translocation factors | Gudkov, 1997 | |||
| Elongation factor Tu, tuf, Mycobacterium fortuitum | K0VK30_MYCFO | 44 kDa | + | + | + | + | + | + | GPTase activity | Uniprot |
| DivIVA domain containing protein, M. avium subsp paratuberculosis | F7P246_MYCPC | 28 kDa | + | + | + | + | Cell shape maintenance | He and de Buck, 2010 | ||
| Chaperone protein DnaK*, Mycobacterium tuberculosis | A2VF50_MYCTX | 67 kDa | + | + | + | + | + | + | Highly antigenic and act as co-repressor for heat shock protein transcription repressor (hspR) | Das Gupta et al., 2008 |
| Aconitate hydratase, Mycobacterium. sylvaticum | V7KF43_MYCAV | 102 kDa | + | + | + | + | Unknown function | Uniprot | ||
| Elongation factor Tu, Mycobacterium avium, | EFTU_MYCA1 | 44 kDa | + | + | + | + | GTPase activity | Uniprot | ||
| Secreted antigen 85-c fbpC*, Mycobacterium bovis | A1KEV0_MYCBP | 37 kDa | + | + | Proteins of antigen 85 are responsible for high affinity of mycobacteria | Uniprot | ||||
| Malate synthétase, Mycobacterium avium | MASZ_MYCA1 | 80 kDa | + | + | Enzyme of glyoxylate shunt | Kumar and Bhakuni, 2011 | ||||
| Diacylglycerol acyltransferase/mycolyltransferase Ag85B, Mycobacterium smegmatis | A85B_MYCS2 | 35 kDa | + | + | Proteins of antigen 85 are responsible for high affinity of mycobacteria | Uniprot | ||||
| Probable glycéraldéhyde 3-phosphate déhydrogénase, Mycobacterium bovis BCG | A1KIM5_MYCBP | 36 kDa | + | + | Phases of glycolysis | Uniprot | ||||
| Iron-dependent repressor IdeR, Mycobacterium avium | A0QIP3_MYCA1 | 25 kDa | + | + | DNA binding transcription factor | Uniprot | ||||
| Putative ESAT-6 like protein 5, esxN *, Mycobacterium bovis (strain BCG/Pasteur 1173P2) | A1KJK3_MYCBP | 10 kDa | + | + | Reported to induce T-cell response | Deng et al., 2014 | ||||
| DNA-directed RNA polymerase subunit beta', rpoC, Mycobacterium tuberculosis | S5ES53_MYCTX | 147 kDa | + | + | Catalyzes the transcription of DNA into RNA | Uniprot | ||||
| 30S ribosomal protein, S4, Mycobacterium fortuitum subsp fortuitum DSM 46621 | KOV712_MYCFO | 53 kDa | + | + | + | Binds directly to 16R rRNA, Essential in invitro growth of Mycobacterium tuberculosis | Griffin et al., 2011 | |||
| 6 kDa early secretory antigenic target esxA (Esat-6)*, Mycobacterium tuberculosis | A2VMQ0_MYCTX | 10 kDa | + | + | Immunodominant antigen involved in secretion, complex formation, immunogenicity and virulence | Brodin et al., 2005 | ||||
| ATP synthase subunit beta, Mycobacterium avium | A0QCX8|ATPB_MYCA1 | 53 kDa | + | + | + | + | + | + | Produces ATP from ADP in the presence of a proton gradient across the membrane. The catalytic sites are hosted primarily by the beta subunits | Uniprot |
| DNA-directed RNA polymerase subunit alpha, rpoA, Mycobacterium kansasii ATCC 12478 | U5WSX2|U5WSX2_MYCKA | 38 kDa | + | + | + | + | + | Catalyzes the transcription of DNA into RNA | Uniprot | |
| Forkhead-associated protein, Mycobacterium avium (strain 104) | A0QGN6|A0QGN6_MYCA1 | 17 kDa | + | + | + | Function is unknown | ||||
| Universal stress protein, Mycobacterium kansasii ATCC 12478 | U5WXR9|U5WXR9_MYCKA | 15 kDa | + | + | Response to stress | Uniprot |
+, Proteins identified in the respective PPDs; Uniprot, Uniprot database (www.uniprot.org),
*
Previously characterized immunogenic proteins of MTBC.