Cloning into pGREG526 |
|
SkATF1-F |
CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACATCTTCAAAAGCCTCCTCATAC |
SkATF1-R |
GCGTGACATAACTAATTACATGACTCGAGGTCGACGCCTAAGGAATGACGATACC |
SuATF1-F |
CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAACAAAACCATAACCGAATACG |
SuATF1-R |
GCGTGACATAACTAATTACATGACTCGAGGTCGACCGGCTAAAAGAACGATACAA |
ScATF1-F |
CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAGGTACTCATCGTAAAAGATTGC |
ScATF1-R |
GCGTGACATAACTAATTACATGACTCGAGGTCGACTAACCAACCAAAGCCGAG |
SkATF2-F |
CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACATTATCACCAGACGGCTCAC |
SkATF2-R |
GCGTGACATAACTAATTACATGACTCGAGGTCGACGCTCTGTCCGATACACCG |
SuATF2-F |
CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAATCACCAAAGTAACCACCAT |
SuATF2-R |
GCGTGACATAACTAATTACATGACTCGAGGTCGACATACCGCTTCCTTGCTGT |
ScATF2-F |
CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAAGGAAGCACGTCAGAAAAAG |
ScATF2-R |
GCGTGACATAACTAATTACATGACTCGAGGTCGACGCTCTGTCCGATACACTGC |
ATF1/ATF2
deletion cassettes
|
|
TaATF1-NAT1-F |
ATGAATGAAATCGATGAGAAAAATCAGGCGCCCGTGCAACGGTGTTTAGGTCGATGCCATC |
TaATF1-NAT1-R |
CTAAGGGCCTAAAAGGAGAGCTTTATAAATGGAGCAAAGCGGATGGCGGCGTTAGTATCG |
TaATF2-NAT1-F |
ATGGAAGATATAGAAGGATACGAACCACATATCACTCAAGGGTGTTTAGGTCGATGCCATC |
TaATF2-NAT1-R |
TTAAAGCGACGCAAATTCGCCGATGGTTTGGTAGAAGAGCGGATGGCGGCGTTAGTATCG |
Integration fragments |
|
pGSkATF1f |
CTTCATCAGTATCACAAATACCATCAATTTATCAGCTCTCATGACTAAAATCAGCGAAGAG |
pGSuATF1f |
CTTCATCAGTATCACAAATACCATCAATTTATCAGCTCTCATGAATACCTATAGTGAAAA |
pGScATF1f |
CTTCATCAGTATCACAAATAC |
pG-ATF1-R |
TCATATTGTCGAATAATATCAGTCAAGCATCATGTGAGATCTCACTATAGGGCGAATTGG |
pGSkATF2f |
CGAATAATAACTTCAGCAATAAAAATTGTCCAGGTTAATTATGGATGATATAGAGGAATAC |
pGSuATF2f |
CGAATAATAACTTCAGCAATAAAAATTGTCCAGGTTAATTATGGATAGTTTAGAGGAATAC |
pGScATF2f |
CGAATAATAACTTCAGCAAT |
pG-ATF2-R |
TCGGCCGAGCTATACGAAGGCCCGCTACGGCAGTATCGCACTCACTATAGGGCGAATTGG |
Integration of
TDH3
promoter
|
|
pG526-TDH3p-Fa |
GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCAGTTCGAGTTTATCATTATC |
pG-TDH3p-CF1-R |
CTGATAAATTGATGGTATTTGTGATACTGATGAAGCGAAACTAAGTTCTTGGTGTT |
pG-TDH3p-KF1-R |
GGTAGATTGATGATATTTGTTATACTGATGAGTGGCGAAACTAAGTTCTTGGTGTT |
pG-TDH3p-UF1-R |
GTTGGATTGATGGTTTTGTTAAACTGCTAAATTGGCGAAACTAAGTTCTTGGTGTT |
pG-TDH3p-CF2-R |
CCTGGACAATTTTTATTGCTGAAGTTATTATTCGTCGAAACTAAGTTCTTGGTGTT |
pG-TDH3p-KF2-R |
CGCAATTTTTTGTTCGTTTGAAGATCTAGTAGAGCCGAAACTAAGTTCTTGGTGTT |
pG-TDH3p-UF2-R |
GCAGTTTTTTGTTCTGAAACCTATTGTTCGTTTGTCGAAACTAAGTTCTTGGTGTT |
Diagnostic |
|
T73ATF1-UF |
GGTACTCATCGTAAAAGATTGC |
T73ATF2-UF |
AGGAAGCACGTCAGAAAAAG |
K2 |
GGGACAATTCAACGCGTCTG |
K3 |
CCTCGACATCATCTGCCC |
SkATF1-R1 |
GCCTAAGGAATGACGATACC |
SuATF1-R1 |
CGGCTAAAAGAACGATACAA |
ScATF1-R1 |
TTTGTGATACTGATGAAGTGCCG |
SkATF2-R1 |
GCTCTGTCCGATACACCG |
SuATF2-R1 |
ATACCGCTTCCTTGCTGT |
ScATF2-R1 |
GCTCTGTCCGATACACTGC |