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. 2016 Jun 7;7:897. doi: 10.3389/fmicb.2016.00897

Table 2.

The primers used in this study.

Primer Sequence 5′–3′
Cloning into pGREG526
   SkATF1-F CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACATCTTCAAAAGCCTCCTCATAC
   SkATF1-R GCGTGACATAACTAATTACATGACTCGAGGTCGACGCCTAAGGAATGACGATACC
   SuATF1-F CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAACAAAACCATAACCGAATACG
   SuATF1-R GCGTGACATAACTAATTACATGACTCGAGGTCGACCGGCTAAAAGAACGATACAA
   ScATF1-F CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAGGTACTCATCGTAAAAGATTGC
   ScATF1-R GCGTGACATAACTAATTACATGACTCGAGGTCGACTAACCAACCAAAGCCGAG
   SkATF2-F CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACATTATCACCAGACGGCTCAC
   SkATF2-R GCGTGACATAACTAATTACATGACTCGAGGTCGACGCTCTGTCCGATACACCG
   SuATF2-F CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAATCACCAAAGTAACCACCAT
   SuATF2-R GCGTGACATAACTAATTACATGACTCGAGGTCGACATACCGCTTCCTTGCTGT
   ScATF2-F CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAAGGAAGCACGTCAGAAAAAG
   ScATF2-R GCGTGACATAACTAATTACATGACTCGAGGTCGACGCTCTGTCCGATACACTGC
ATF1/ATF2 deletion cassettes
   TaATF1-NAT1-F ATGAATGAAATCGATGAGAAAAATCAGGCGCCCGTGCAACGGTGTTTAGGTCGATGCCATC
   TaATF1-NAT1-R CTAAGGGCCTAAAAGGAGAGCTTTATAAATGGAGCAAAGCGGATGGCGGCGTTAGTATCG
   TaATF2-NAT1-F ATGGAAGATATAGAAGGATACGAACCACATATCACTCAAGGGTGTTTAGGTCGATGCCATC
   TaATF2-NAT1-R TTAAAGCGACGCAAATTCGCCGATGGTTTGGTAGAAGAGCGGATGGCGGCGTTAGTATCG
Integration fragments
   pGSkATF1f CTTCATCAGTATCACAAATACCATCAATTTATCAGCTCTCATGACTAAAATCAGCGAAGAG
   pGSuATF1f CTTCATCAGTATCACAAATACCATCAATTTATCAGCTCTCATGAATACCTATAGTGAAAA
   pGScATF1f CTTCATCAGTATCACAAATAC
   pG-ATF1-R TCATATTGTCGAATAATATCAGTCAAGCATCATGTGAGATCTCACTATAGGGCGAATTGG
   pGSkATF2f CGAATAATAACTTCAGCAATAAAAATTGTCCAGGTTAATTATGGATGATATAGAGGAATAC
   pGSuATF2f CGAATAATAACTTCAGCAATAAAAATTGTCCAGGTTAATTATGGATAGTTTAGAGGAATAC
   pGScATF2f CGAATAATAACTTCAGCAAT
   pG-ATF2-R TCGGCCGAGCTATACGAAGGCCCGCTACGGCAGTATCGCACTCACTATAGGGCGAATTGG
Integration of TDH3 promoter
   pG526-TDH3p-Fa GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCAGTTCGAGTTTATCATTATC
   pG-TDH3p-CF1-R CTGATAAATTGATGGTATTTGTGATACTGATGAAGCGAAACTAAGTTCTTGGTGTT
   pG-TDH3p-KF1-R GGTAGATTGATGATATTTGTTATACTGATGAGTGGCGAAACTAAGTTCTTGGTGTT
   pG-TDH3p-UF1-R GTTGGATTGATGGTTTTGTTAAACTGCTAAATTGGCGAAACTAAGTTCTTGGTGTT
   pG-TDH3p-CF2-R CCTGGACAATTTTTATTGCTGAAGTTATTATTCGTCGAAACTAAGTTCTTGGTGTT
   pG-TDH3p-KF2-R CGCAATTTTTTGTTCGTTTGAAGATCTAGTAGAGCCGAAACTAAGTTCTTGGTGTT
   pG-TDH3p-UF2-R GCAGTTTTTTGTTCTGAAACCTATTGTTCGTTTGTCGAAACTAAGTTCTTGGTGTT
Diagnostic
   T73ATF1-UF GGTACTCATCGTAAAAGATTGC
   T73ATF2-UF AGGAAGCACGTCAGAAAAAG
   K2 GGGACAATTCAACGCGTCTG
   K3 CCTCGACATCATCTGCCC
   SkATF1-R1 GCCTAAGGAATGACGATACC
   SuATF1-R1 CGGCTAAAAGAACGATACAA
   ScATF1-R1 TTTGTGATACTGATGAAGTGCCG
   SkATF2-R1 GCTCTGTCCGATACACCG
   SuATF2-R1 ATACCGCTTCCTTGCTGT
   ScATF2-R1 GCTCTGTCCGATACACTGC