Table 2.
The primers used in this study.
| Primer | Sequence 5′–3′ |
|---|---|
| Cloning into pGREG526 | |
| SkATF1-F | CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACATCTTCAAAAGCCTCCTCATAC |
| SkATF1-R | GCGTGACATAACTAATTACATGACTCGAGGTCGACGCCTAAGGAATGACGATACC |
| SuATF1-F | CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAACAAAACCATAACCGAATACG |
| SuATF1-R | GCGTGACATAACTAATTACATGACTCGAGGTCGACCGGCTAAAAGAACGATACAA |
| ScATF1-F | CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAGGTACTCATCGTAAAAGATTGC |
| ScATF1-R | GCGTGACATAACTAATTACATGACTCGAGGTCGACTAACCAACCAAAGCCGAG |
| SkATF2-F | CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACATTATCACCAGACGGCTCAC |
| SkATF2-R | GCGTGACATAACTAATTACATGACTCGAGGTCGACGCTCTGTCCGATACACCG |
| SuATF2-F | CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAATCACCAAAGTAACCACCAT |
| SuATF2-R | GCGTGACATAACTAATTACATGACTCGAGGTCGACATACCGCTTCCTTGCTGT |
| ScATF2-F | CCTAGTACGGATTAGAAGCCGCCGAGCGGGTGACAAGGAAGCACGTCAGAAAAAG |
| ScATF2-R | GCGTGACATAACTAATTACATGACTCGAGGTCGACGCTCTGTCCGATACACTGC |
| ATF1/ATF2 deletion cassettes | |
| TaATF1-NAT1-F | ATGAATGAAATCGATGAGAAAAATCAGGCGCCCGTGCAACGGTGTTTAGGTCGATGCCATC |
| TaATF1-NAT1-R | CTAAGGGCCTAAAAGGAGAGCTTTATAAATGGAGCAAAGCGGATGGCGGCGTTAGTATCG |
| TaATF2-NAT1-F | ATGGAAGATATAGAAGGATACGAACCACATATCACTCAAGGGTGTTTAGGTCGATGCCATC |
| TaATF2-NAT1-R | TTAAAGCGACGCAAATTCGCCGATGGTTTGGTAGAAGAGCGGATGGCGGCGTTAGTATCG |
| Integration fragments | |
| pGSkATF1f | CTTCATCAGTATCACAAATACCATCAATTTATCAGCTCTCATGACTAAAATCAGCGAAGAG |
| pGSuATF1f | CTTCATCAGTATCACAAATACCATCAATTTATCAGCTCTCATGAATACCTATAGTGAAAA |
| pGScATF1f | CTTCATCAGTATCACAAATAC |
| pG-ATF1-R | TCATATTGTCGAATAATATCAGTCAAGCATCATGTGAGATCTCACTATAGGGCGAATTGG |
| pGSkATF2f | CGAATAATAACTTCAGCAATAAAAATTGTCCAGGTTAATTATGGATGATATAGAGGAATAC |
| pGSuATF2f | CGAATAATAACTTCAGCAATAAAAATTGTCCAGGTTAATTATGGATAGTTTAGAGGAATAC |
| pGScATF2f | CGAATAATAACTTCAGCAAT |
| pG-ATF2-R | TCGGCCGAGCTATACGAAGGCCCGCTACGGCAGTATCGCACTCACTATAGGGCGAATTGG |
| Integration of TDH3 promoter | |
| pG526-TDH3p-Fa | GAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCAGTTCGAGTTTATCATTATC |
| pG-TDH3p-CF1-R | CTGATAAATTGATGGTATTTGTGATACTGATGAAGCGAAACTAAGTTCTTGGTGTT |
| pG-TDH3p-KF1-R | GGTAGATTGATGATATTTGTTATACTGATGAGTGGCGAAACTAAGTTCTTGGTGTT |
| pG-TDH3p-UF1-R | GTTGGATTGATGGTTTTGTTAAACTGCTAAATTGGCGAAACTAAGTTCTTGGTGTT |
| pG-TDH3p-CF2-R | CCTGGACAATTTTTATTGCTGAAGTTATTATTCGTCGAAACTAAGTTCTTGGTGTT |
| pG-TDH3p-KF2-R | CGCAATTTTTTGTTCGTTTGAAGATCTAGTAGAGCCGAAACTAAGTTCTTGGTGTT |
| pG-TDH3p-UF2-R | GCAGTTTTTTGTTCTGAAACCTATTGTTCGTTTGTCGAAACTAAGTTCTTGGTGTT |
| Diagnostic | |
| T73ATF1-UF | GGTACTCATCGTAAAAGATTGC |
| T73ATF2-UF | AGGAAGCACGTCAGAAAAAG |
| K2 | GGGACAATTCAACGCGTCTG |
| K3 | CCTCGACATCATCTGCCC |
| SkATF1-R1 | GCCTAAGGAATGACGATACC |
| SuATF1-R1 | CGGCTAAAAGAACGATACAA |
| ScATF1-R1 | TTTGTGATACTGATGAAGTGCCG |
| SkATF2-R1 | GCTCTGTCCGATACACCG |
| SuATF2-R1 | ATACCGCTTCCTTGCTGT |
| ScATF2-R1 | GCTCTGTCCGATACACTGC |