Fig. 3.

Effects of α-M on extracellular H₂O₂ release in α-synuclein-stimulated microglia. a Microglial cells were stimulated by α-synuclein with or without α-M. b, c After pretreat with NOX-1-pcDNA3.0 for 24 h, H₂O₂ concentrations in the medium were measured at the indicated points. The concentration of H₂O₂ and superoxide in cultural supernatants were measured by Amplex Red or Wst-1, respectively. ***p < 0.001 versus control, ^# p < 0.05 versus α-synuclein, ^## p < 0.01 versus α-synuclein and ^### p < 0.001 versus α-synuclein