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. 2016 Jan 18;73(13):2565–2581. doi: 10.1007/s00018-016-2131-3

Fig. 3.

Fig. 3

The C-termini of two TPC1 monomers interact with each other. a Confocal fluorescence overlay images of the Venus (yellow) and chlorophyll (red) signals of a tobacco cell co-expressing TPC1 fused to Venus-Ct (TPC1-VC) and TPC1 fused to Venus-Nt (TPC1-VN), shown at two different magnifications (scales represent 5 μm). The BiFC signals emerge from the tonoplast. b Whole-vacuolar current response of a tpc1-2 cell co-expressing the same constructs as in (a). c Confocal fluorescence overlay image of the YFP (yellow) and chlorophyll (red) signals of a tobacco cell co-expressing TPC1-3LP-VC and TPC1-3LP-VN (scales represent 5 μm). The BiFC signals emerge from endomembranes and tonoplast. d Fluorescence signal intensities of BiFC experiments in relative units, determined from leaf disks with a fluorescence reader. VC-TPC1 and VN-TPC1 (n = 23), TPC1-VC and VN-TPC1 (n = 23), VC-TPC1 and TPC1-VN (n = 23), TPC1-VC and TPC1-VN (n = 38), TPC1-3LP-VC and TPC1-3LP-VN (n = 11). As negative controls leaf disks only expressing the helper strain p19 (n = 28) and not infiltrated leaves (n = 39) were used