Figure 1. PRA/PRB show differential interaction with AP-1 transcription factors and transcriptional co-repressor proteins p54^nrb/mSIN3A.

In situ proximity ligation assay (PLA) of PRs with Jun and Fos family members and co-repressor proteins; p54^nrb and mSIN3A. PRA and PRB stable transfected hTERT-HM cells were treated with 100 nM progesterone (P4) for 2 h and then subjected to PLA. (a) Representative pictures and (b) signal analysis (average nuclear signals/cell from three fields), pooled from three independent experiments are shown. Two-way analysis of variance followed by bonferroni post-tests show significant differences between PRA and PRB in the interaction of cJUN (P<0.001, denoted as ***), Fra1 (P<0.001), Fra2 (P<0.01, denoted as **), p54 (P<0.05, denoted as *), and mSIN3A (P<0.01). Data represents mean±s.d., n=3. Scale bar, 20 μm.