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. 2016 May 26;7:11704. doi: 10.1038/ncomms11704

Figure 6. Hsp90 modulates expression of HSF1.

Figure 6

(a) Depletion of HSP90 increases the binding signal of Hsf1 in the absence of heat shock compared with the wild type. Blue represents untreated, red represents heat shocked cells. (b) Expression of HSF1, measured by RNA-seq, increases upon HSP90 depletion in the absence of heat shock. HSF1 expression in wild-type (SN95, WT) or tetO-HSP90/hsp90Δ strain in the absence (−) or presence (+) of 20 μg ml−1 doxycycline to repress HSP90 expression. Data represent mean values ± s.d. from three independent biological replicates (c) Heat induction of HSF1 expression in wild-type (dark red) versus tetO-HSP90/hsp90Δ (light red) cells over a 10, 30 or 60 min 30–42 °C heat shock.