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. 2016 May 27;7:11667. doi: 10.1038/ncomms11667

Figure 7. CsrA and FliW influence flaA mRNA localization to the poles.

Figure 7

(a) RNA-FISH analysis (Left: confocal microscopy images; Right: averaged fluorescence intensity along the long axis based on 10 cells) of 16S rRNA (green) and flaA mRNA (red) in C. jejuni NCTC11168 WT, ΔcsrA, ΔfliW, ΔcsrAfliW and ΔfliA strains in mid-log phase. FITC and Cy5 channels were merged in the microcopy images in the third lanes (scale bar, 1 μm). (b) Super-resolution microscopy imaging of flaA mRNA RNA-FISH (14 Cy5-labelled oligos) in the indicated C. jejuni strains using dSTORM imaging. Cell boundaries from bright-field images are depicted by white dotted lines.